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卵泡 1

复方口服避孕药 1

无活性药间期 1

激素 1

经阴道超声；排卵监测；小卵泡排卵；未破裂卵泡黄素化；促排卵治疗 1

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MicroRNA-148b promotes proliferation of hair follicle cells by targeting

Wanbao YANG,Qinqun LI,Bo SU,Mei YU

《农业科学与工程前沿（英文）》 2016年第3卷第1期页码 72-80 doi: 10.15302/J-FASE-2016089

摘要： MicroRNAs (miRNAs), small non-coding RNAs, are involved in many aspects of biological processes. Previous studies have indicated that miRNAs are important for hair follicle development and growth. In our study, we found by qRT-PCR that miR-148b was significantly upregulated in sheep wool follicle bulbs in anagen phase compared with the telogen phase of the hair follicle cycle. Overexpression of miR-148b promoted proliferation of both HHDPC and HHGMC. By using the TOPFlash system we demonstrated that miR-148b could activate Wnt/β-catenin pathway and , , and were consistently upregulated accordingly. Furthermore, transcript factor nuclear factor of activated T cells type 5 ( ) and were predicted to be the target of miR-148b and this was substantiated using a Dual-Luciferase reporter system. Subsequently was further identified as the target of miR-148b using western blotting. These results were considered to indicate that miR-148b could activate the Wnt/β-catenin signal pathway by targeting to promote the proliferation of human hair follicle cells.

关键词： miR-148b hair follicle proliferation NFAT5 Wnt10b

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Role of Wnt and Notch signaling in regulating hair cell regeneration in the cochlea

null

《医学前沿（英文）》 2016年第10卷第3期页码 237-249 doi: 10.1007/s11684-016-0464-9

摘要：

Sensory hair cells in the inner ear are responsible for sound recognition. Damage to hair cells in adult mammals causes permanent hearing impairment because these cells cannot regenerate. By contrast, newborn mammals possess limited regenerative capacity because of the active participation of various signaling pathways, including Wnt and Notch signaling. The Wnt and Notch pathways are highly sophisticated and conserved signaling pathways that control multiple cellular events necessary for the formation of sensory hair cells. Both signaling pathways allow resident supporting cells to regenerate hair cells in the neonatal cochlea. In this regard, Wnt and Notch signaling has gained increased research attention in hair cell regeneration. This review presents the current understanding of the Wnt and Notch signaling pathways in the auditory portion of the inner ear and discusses the possibilities of controlling these pathways with the hair cell fate determiner Atoh1 to regulate hair cell regeneration in the mammalian cochlea.

关键词： inner ear cochlea hair cell regeneration Wnt Notch signaling pathways

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Regeneration of hair cells in the mammalian vestibular system

null

《医学前沿（英文）》 2016年第10卷第2期页码 143-151 doi: 10.1007/s11684-016-0451-1

摘要：

Hair cells regenerate throughout the lifetime of non-mammalian vertebrates, allowing these animals to recover from hearing and balance deficits. Such regeneration does not occur efficiently in humans and other mammals. Thus, balance deficits become permanent and is a common sensory disorder all over the world. Since Forge and Warchol discovered the limited spontaneous regeneration of vestibular hair cells after gentamicin-induced damage in mature mammals, significant efforts have been exerted to trace the origin of the limited vestibular regeneration in mammals after hair cell loss. Moreover, recently many strategies have been developed to promote the hair cell regeneration and subsequent functional recovery of the vestibular system, including manipulating the Wnt, Notch and Atoh1. This article provides an overview of the recent advances in hair cell regeneration in mammalian vestibular epithelia. Furthermore, this review highlights the current limitations of hair cell regeneration and provides the possible solutions to regenerate functional hair cells and to partially restore vestibular function.

关键词： utricle hair cell regeneration Atoh1 Notch Wnt

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Behavioral methods for the functional assessment of hair cells in zebrafish

null

《医学前沿（英文）》 2017年第11卷第2期页码 178-190 doi: 10.1007/s11684-017-0507-x

摘要：

Zebrafish is an emerging animal model for studies on auditory system. This model presents high comparability with humans, good accessibility to the hearing organ, and high throughput capacity. To better utilize this animal model, methodologies need to be used to quantify the hearing function of the zebrafish. Zebrafish displays a series of innate and robust behavior related to its auditory function. Here, we reviewed the advantage of using zebrafish in auditory research and then introduced three behavioral tests, as follows: the startle response, the vestibular-ocular reflex, and rheotaxis. These tests are discussed in terms of their physiological characteristics, up-to-date technical development, and apparatus description. Test limitation and areas to improve are also introduced. Finally, we revealed the feasibility of these applications in zebrafish behavioral assessment and their potential in the high-throughput screening on hearing-related genes and drugs.

关键词： zebrafish (Danio rerio) behavior auditory startle response vestibular-ocular reflex rheotaxis

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Relationship between selenium concentration in child hair and the distribution of Kashin-Beck Disease

LI Shunjiang, YANG Linsheng, WANG Wuyi, LI Yonghua, LI Hairong, XIRAO Ruodeng

《医学前沿（英文）》 2007年第1卷第2期页码 223-225 doi: 10.1007/s11684-007-0043-1

摘要： The focus of this study is to determine the relationship between selenium (Se) concentrations in child hair and the distribution of Kashin-Beck disease (KBD) in Tibet. The hair of children in typical KBD-affected areas and non-disease areas was collected, and its Se concentrations were measured by atomic fluorescent spectrometry. The Se concentrations in child hair were (0.13±0.04) and (0.18±0.07) µg/g respectively in KBD areas and non-disease areas respectively, which are significantly different. In areas supplemented with Se in Tibet, the Se concentration of hair in KBD- affected areas was higher than that in non-diseased areas, being (0.28±0.03) and (0.18±0.04) µg/g respectively. There is a close relationship between the Se concentrations in hair and KBD distribution. Se supplement increase the Se concentrations in the human body. Appropriate Se supplementation is important to prevent KBD in Tibet.

关键词： different relationship non-disease selenium KBD distribution

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Apigenin alleviates neomycin-induced oxidative damage via the Nrf2 signaling pathway in cochlear hair

《医学前沿（英文）》 2022年第16卷第4期页码 637-650 doi: 10.1007/s11684-021-0864-3

摘要： Oxidative stress plays an important role in the pathogenesis of aminoglycoside-induced hearing loss and represents a promising target for treatment. We tested the potential effect of apigenin, a natural flavonoid with anticancer, anti-inflammatory, and antioxidant activities, on neomycin-induced ototoxicity in cochlear hair cells in vitro. Results showed that apigenin significantly ameliorated the loss of hair cells and the accumulation of reactive oxygen species upon neomycin injury. Further evidence suggested that the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway was activated by apigenin treatment. Disruption of the Nrf2 axis abolished the effects of apigenin on the alleviation of oxidative stress and subsequent apoptosis of hair cells. This study provided evidence of the protective effect of apigenin on cochlear hair cells and its underlying mechanism.

关键词： apigenin aminoglycosides ototoxicity oxidative stress Nrf2 signaling pathway

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Atypical pituitary hormone–target tissue axis

《医学前沿（英文）》 2023年第17卷第1期页码 1-17 doi: 10.1007/s11684-022-0973-7

摘要： A long-held belief is that pituitary hormones bind to their cognate receptors in classical target glands to actuate their manifold functions. However, a number of studies have shown that multiple types of pituitary hormone receptors are widely expressed in non-classical target organs. Each pituitary gland-derived hormone exhibits a wide range of nonconventional biological effects in these non-classical target organs. Herein, the extra biological functions of pituitary hormones, thyroid-stimulating hormone, follicle-stimulating hormone, luteinizing hormone, adrenocorticotrophic hormone, and prolactin when they act on non-classical organs were summarized, defined by the novel concept of an “atypical pituitary hormone–target tissue axis.” This novel proposal explains the pathomechanisms of abnormal glucose and lipid metabolism, obesity, hypertension, fatty liver, and atherosclerosis while offering a more comprehensive and systematic insights into the coordinated regulation of environmental factors, genetic factors, and neuroendocrine hormones on human biological functions. The continued exploration of the physiology of the “atypical pituitary hormone–target tissue axis” could enable the identification of novel therapeutic targets for metabolic diseases.

关键词： thyroid-stimulating hormone follicle-stimulating hormone luteinizing hormone adrenocorticotrophic hormone prolactin

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Loss of liver kinase B1 causes planar polarity defects in cochlear hair cells in mice

null

《医学前沿（英文）》 2016年第10卷第4期页码 481-489 doi: 10.1007/s11684-016-0494-3

摘要：

The tumor suppressor gene liver kinase B1 (LKB1), also called STK11, encodes a serine/threonine kinase. LKB1 plays crucial roles in cell differentiation, proliferation, and polarity. In this study, LKB1 conditional knockout mice (LKB1^Pax2 CKO mice) were generated using Pax2-Cre mice to investigate the function of LKB1 in inner ear hair cells during early embryonic period. LKB1^Pax2 CKO mice died perinatally. Immunofluorescence and scanning electron microscopy revealed that stereociliary bundles in LKB1^Pax2 CKO mice were clustered and misoriented, respectively. Moreover, ectopic distribution of kinocilium bundles resulting from abnormal migration of kinocilium was observed in the mutant mice. The orientation of stereociliary bundles and the migration of kinocilia are critical indicators of planar cell polarity (PCP) of hair cells. LKB1 deficiency in LKB1^Pax2 CKO mice thus disrupted hair cell planar polarity during embryonic development. Our results suggest that LKB1 is required in PCP formation in cochlear hair cells in mice.

关键词： LKB1 stereociliary bundles kinocilium planar cell polarity hearing mice

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Identification and selection of resistance to

Lizhen ZHU, Jianying LI, Zhongping XU, Hakim MANGHWAR, Sijia LIANG, Suli LI, Muna ALARIQI, Shuangxia JIN, Xianlong ZHANG

《农业科学与工程前沿（英文）》 2018年第5卷第2期页码 236-252 doi: 10.15302/J-FASE-2018223

摘要： Plants have developed sophisticated systems to cope with herbivore challenge, including morphological barriers and secondary metabolites to reduce damage. In this study, 550 genotypes were evaluated for whitefly ( ) resistance in five experiments including two in the field and three in the greenhouse, with 23 resistant and 19 susceptible genotypes selected. Whitefly-resistance index determination showed that a leaf having a high density of hairs had resistance to whitfly egg/nymph production. Longer leaf hairs were also important for resistance. This study revealed that okra shaped leaves reduced adult whitefly oviposition preference, while glabrous leaves and high hair density helped not only in the reduction of the adults but also decreased oviposition preference. Gossypol was also observed to be involved in the reduction of adult whitefly development and/or survival.

关键词： Bemisia tabaci Gossypium genotypes gossypol leaf hair density leaf hair length

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Alternative splicing of inner-ear-expressed genes

null

《医学前沿（英文）》 2016年第10卷第3期页码 250-257 doi: 10.1007/s11684-016-0454-y

摘要：

Alternative splicing plays a fundamental role in the development and physiological function of the inner ear. Inner-ear-specific gene splicing is necessary to establish the identity and maintain the function of the inner ear. For example, exon 68 of Cadherin 23 (Cdh23) gene is subject to inner-ear-specific alternative splicing, and as a result, Cdh23(+68) is only expressed in inner ear hair cells. Alternative splicing along the tonotopic axis of the cochlea contributes to frequency tuning, particularly in lower vertebrates, such as chickens and turtles. Differential splicing of Kcnma1, which encodes for the α subunit of the Ca²⁺-activated K⁺ channel (BK channel), has been suggested to affect the channel gating properties and is important for frequency tuning. Consequently, deficits in alternative splicing have been shown to cause hearing loss, as we can observe in Bronx Waltzer (bv) mice and Sfswap mutant mice. Despite the advances in this field, the regulation of alternative splicing in the inner ear remains elusive. Further investigation is also needed to clarify the mechanism of hearing loss caused by alternative splicing deficits.

关键词： alternative splicing inner ear hearing loss hair cells

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is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice

Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao

《医学前沿（英文）》 2019年第13卷第6期页码 690-704 doi: 10.1007/s11684-018-0638-8

摘要： encodes the taperin protein, which is concentrated in the tapered region of hair cell stereocilia in the inner ear. In humans, mutations cause autosomal recessive nonsyndromic deafness (DFNB79) by an unknown mechanism. To determine the role of in hearing, we generated -null mice by clustered regularly interspaced short palindromic repeat/Cas9 genome-editing technology from a CBA/CaJ background. We observed significant hearing loss and progressive degeneration of stereocilia in the outer hair cells of -null mice starting from postnatal day 30. Transmission electron microscopy images of stereociliary bundles in the mutant mice showed some stereociliary rootlets with curved shafts. The central cores of the stereociliary rootlets possessed hollow structures with surrounding loose peripheral dense rings. Radixin, a protein expressed at stereocilia tapering, was abnormally dispersed along the stereocilia shafts in null mice. The expression levels of radixin and -actin significantly decreased. We propose that is critical to the retention of the integrity of the stereociliary rootlet. Loss of in -null mice caused the disruption of the stereociliary rootlet, which resulted in damage to stereociliary bundles and hearing impairments. The generated -null mice are ideal models of human hereditary deafness DFNB79.

关键词： TPRN stereocilia stereociliary rootlet actin filament CRISPR/Cas9 hearing

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Expression pattern and regulation of head-to-head genes

Xinxing CUI,Chunhong YANG,Li KANG,Guiyu ZHU,Qingqing WEI,Yunliang JIANG

《农业科学与工程前沿（英文）》 2014年第1卷第2期页码 130-136 doi: 10.15302/J-FASE-2014013

摘要： Vacuolar protein sorting 36 (VPS36), a protein primarily known for its role in the Endosomal Sorting Complex Required for Transport pathway, has recently been shown to be linked to chicken reproduction. Previous research showed that is significantly downregulated in sexually mature chicken ovaries compared to immature ones. In this study, using real-time quantitative RT-PCR, we investigated the expression pattern of and its head-to-head gene mRNA in chicken follicles. Small white follicles were found to have significantly higher expression of and mRNA than any other sized follicles ( <0.05). The expression of and mRNA were detected in both granulosa and theca layers of pre-ovulatory follicles, the expression of in theca layers was slightly higher than in granulosa cells. Treatment of small yellow follicles with follicle-stimulating hormone and estradiol resulted in a marked decrease of both and mRNA ( <0.05); however, progesterone, transforming growth factor-β 1 and luteinizing hormone induced no significant changes in and mRNA expression in these follicles. These results indicate that the head-to-head genes of and exhibit similar expression in chicken follicles and are involved in chicken follicle development.

关键词： chicken Vps36 Ckap2 mRNA follicle

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复方口服避孕药对垂体-卵巢轴激素及卵泡发育的影响

黄培,黄勋彬

《中国工程科学》 2015年第17卷第6期页码 16-20

摘要：

复方口服避孕药（combined oral contraceptives，COCs）由外源性雌孕激素配伍而成，主要是通过高于生理水平的雌孕激素负反馈抑制垂体-卵巢轴，降低内源性促性腺激素和性激素的水平，影响卵泡的发育而避孕。但在周期性的COCs的无活性药间期（hormone-free interval， HFI）会有垂体-卵巢轴活性的恢复，其激素水平的变化及卵泡发育的情况与雌激素的剂量、孕激素的剂型、HFI的改变相关。

关键词：复方口服避孕药无活性药间期激素卵泡

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两种特殊类型排卵障碍的超声监测和促排卵治疗疗效观察

刘莉,吴春林,胡雅君

《中国工程科学》 2015年第17卷第6期页码 31-35

摘要：

目的：了解正常排卵、小卵泡排卵以及未破裂卵泡黄素化患者的临床特征及观察后两者经阴道B超再次监排情况和促排卵治疗效果。方法：回顾性分析了正常排卵组（258例）、小卵泡排卵组（65例）及未破裂卵泡黄素化组（27例）患者年龄、排卵前卵泡大小和基础性激素水平差异，观察了后两组排卵障碍患者经阴道B超再次监排情况。并对两次监排结果一致的58例小卵泡排卵和25例未破裂卵泡黄素化患者进行克罗米芬促排卵治疗，分别观察了这两种排卵障碍患者的正常排卵率及临床妊娠率。结果：正常排卵组、小卵泡排卵组和未破裂卵泡黄素化组患者平均年龄、基础性激素水平均无显著性差异。三组间排卵前卵泡最大平均径线有显著性差异（F=117.9，P<0.01）。小卵泡排卵和未破裂卵泡黄素化的发生率分别为18.57 %和7.71 %。两者再次监排后，再发率分别为89.23 %和92.59 %。经克罗米芬促排卵联合黄体支持治疗后，小卵泡排卵组和未破裂卵泡黄素化组正常排卵率分别为70.69 %和80 %，临床妊娠率分别为29.31 %和48 %。结论：年龄、基础性激素水平不能预测是否正常排卵，经阴道B超监测是诊断小卵泡排卵以及未破裂卵泡黄素化的直观方法。不经过治疗两者再发率高，促排卵治疗联合黄体支持是治疗小卵泡排卵以及未破裂卵泡黄素化的有效方法。

关键词：经阴道超声；排卵监测；小卵泡排卵；未破裂卵泡黄素化；促排卵治疗

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标题作者时间类型操作

MicroRNA-148b promotes proliferation of hair follicle cells by targeting

Wanbao YANG,Qinqun LI,Bo SU,Mei YU

期刊论文

Role of Wnt and Notch signaling in regulating hair cell regeneration in the cochlea

null

期刊论文

Regeneration of hair cells in the mammalian vestibular system

null

期刊论文